Protocols (Materials and Methods)
In conducting its research, the Szaniszlo Lab makes use of a wide variety of tools and techniques within the disciplines of chemistry and molecular biology. For the edification of the curious layman and for the reference of the researchers in this lab and elsewhere, common protocols, guidelines, and media recipes are indexed and available on this page.
INDEX:
(to jump to a section, click on the desired selection on the right)

General Laboratory
Molecular Biology Protocols
Plasmids and Plasmid Maps

Chemical Protocols
Media Recipes
Reagents
Buffers
Misc

  General Laboratory
  Maintenance instructions
  Biosafety manual
  Laboratory Equipment Decontamination Form
 

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  Molecular Biology Protocols
  Routine Culture of wild-type Wangiella dermatitidis [ATCC 3410; Exophiala dermatitidis (CBS 527.6)] strain 8656
  DNA isolation (zymolyase) from Wangiella dermatitidis
  DNA isolation (glass beads) from Wangiella dermatitidis
  Genetic Transformation
  Spheroplast fusion in Wangiella dermatitidis
  PCR amplification of fungal chitin synthase homologous sequences
  A spectrophotometric assay for orotidine-5'-monophosphate pyrophosphorylase activity in Wangiella dermatitidis
 

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  Plasmids and Plasmid Maps
  Sequence of hygromycin gene and plasmid map of pCB1004  (PDF)
 

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  Chemical Protocols
  Chitin assay 1
  Chitin assay 2
  Chitin synthase activity assay
  Glucan synthetase assay
 

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  Formulations
  Media Recipes
  MCDB (Modified Czapek Dox) Broth
  Corn Meal Agar
  CDN Agar
  CDY (Czapek Dox Yeast Extract)
  Malt Extract/Peptone (ME/P)
  Minimal Medium (SD)
  YPD Broth
 

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  Reagents
  Phenol prep
  SOS
  5M NaCl
  10% SDS
  5M Potassium acetate
  1M Magnesium chloride
  0.5 M EDTA
  STE
 

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  Buffers
  TEM buffer
  TM buffer
  Gel buffer
  Phosphate buffered saline (PBS)
  Tris HCl
 

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  Misc
  Equipment List
 

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Website established June 24, 1999
University of Texas at Austin

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