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Buffer Recipes TEM buffer
50mM Tris, pH 7.5
1mM EDTA
ß-mercaptoethanol
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the top) TM buffer
50 mM Tris-HCl
2.5 mM MgCl2
pH 7.0l
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the top) Gel buffer
make up 1000 cm3 (TBE)
100 ml 10xTBE
900 ml dH2O
10 µl Ethidium Br.
Gel loading buffer
0.25% Bromophenol Blue
40% (w/v) sucrose in H2O
} store at 4ºC
0.25% xylene cyanol
(Back to
the top) Phosphate
buffered saline (PBS)
10
g NaCl
0.25 g KCl
1.43 g Na2HPO4
0.25 KH2PO4
Make up to 1000cm3 w/ dH2O
Check pH is 7.2
Stir Bar
Autoclave
(Back to
the top) Tris-HCl
1M
12.1 g Tris Base in 80 ml dH2O
pH w/ conc HCl and make final vol = 100 ml w/ dH2O
10mM
0.12 g Tris Base in 80 ml H2O
pH w/ conc HCl and make up to 100 ml w/ dH2O
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