|
Media Recipes MCDB (Modified Czapek Dox) Broth
(Oujezdsky K.B., Grove S.N., and P.J.
Szaniszlo. 1972. Morphological and structural changes during the yeast-to-mold conversion
of Phialophora dermatitidis. J. Bacteriol. 113:468-477.)
Czapek Dox broth (Difco) w/ 1.0% yeast extract (Difco)
This medium is made using pre-prepared Difco Czapek Dox broth which
contains sucrose as a carbon-source.
(Back to
the top)
Corn
Meal Agar
17g Corn Meal Agar (Difco)
10g agar (Difco) CDN
Agar
Into 800 ml distilled H20 add:
10 ml 30% NaNO3
5 ml 20% K2HPO4
5 ml 20% MgSO4:7H2O
5 ml 10% KCl
30 mg glucose/dextrose
Bring to 1 liter and adjust pH to 6.5, then add:
3 mg thiamine / L
5.3g NH4Cl / L
If necessary readjust to pH of 6.5, then add 20g Bacto-agar
CDN is CDY broth in which the yeast extract component was replaced
with 3 mg of thiamine and 5.3 g of NH4Cl for each liter of medium. For determination of
auxotrophy, CDN was supplemented with adenine, arginine, methionine, and uracil as
appropriate.
Solid media were prepared by adding 2% (wt/vol) Bacto agar (Difco)
or 1% (wt/vol) GelRIte (Kelco, San Diego, Calif.) prior to autoclaving.
(Back to
the top) CDY (Czapek Dox Yeast Extract)
Add to 800 ml distilled water
10 ml 30% NaNO3
5 ml 20% K2HPO4
5 ml 20% MgSO4:7H2O
5 ml 10% KCl
30 mg glucose/dextrose
Adjust pH to 6.5
Add 1 g Bacto yeast extract
1 ml fresh FeSO4·7H2O (10ml /10ml)
adjust to 1 L by H20
For solid: add 20 g of agar
CDY (g/l)
Adapted from (Roberts, R.L., and P.J. Szaniszlo. 1978.
Temperature-sensitive multicellular mutants of Wangiella dermatitidis. J.
Bacteriol. 135:622-632.)
30 g/l dextrose,
3 g/l NaNO 3
1 g/l K2HPO4
0.5 g/l MgSO4
Adjust to pH of 6.5
0.01 g/l FeSO 4
Adjust to pH of 6.5
1 g/l yeast extract (Difco)
(Back to
the top) Malt
Extract/Peptone (ME/P)
30 g malt extract
10 g peptone
1 L dd-H20
(Back to
the top) Minimal Medium (SD)
1.7 gm yeast nitrogen base
without amino acids and } Autoclave
without ammonium sulfate
5 gm ammonium sulfate
400 ml H 20
20 gm Bacto-agar
500 ml H20 } Autoclave
After autoclave, mix 2 together and add 50 ml 40% glucose
(Back to
the top) YPD
Broth
10g
Yeast Extract
20 g Poly peptone
20 g Dextrose
1L H20
mix and autoclave
(Back to
the top) |
