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1. Yeast Transformation. a. Large scale high efficiency transformation b. 2 hybrid transformation (Gietz lab protocol) 2. Chromatin immunoprecipitation (ChIP) 3. Yeast DNA mini prep (10 ml) 5. Yeast DNA prep (Large scale 40 ml) 6. Yeast Protein prep for western analysis 7. Yeast plasmid prep for E. coli Transformation Clontech protocol to use with column 8. Yeast plasmid prep for yeast transformation Total DNA isolation using Qiagen method and then do the transformation. 10. RNA isolation (Koshland lab) 11. Haploid yeast cell density 12. DAPI staining of live yeast cells 13. Cell synchronization (using alpha factor) 14. Nocodazole treatment (arrests at G-M phase) 15. Hydroxyl urea arrest 16. FACS analysis using Propidium Iodide 17. Scoring sheets and Grids b. Marker/growth scoring sheet c. Grid for streaking colonies (small plate) d. Streaking grid for bigger plates 21. Making E. coli competent cells (RbCl method) 22. Kinasing Oligos |
JAYARAM LAB |