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Protocols

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 1. Yeast Transformation.

        a. Large scale high efficiency transformation

        b. 2 hybrid transformation (Gietz lab protocol)

        c. Fast and easy method

2. Chromatin immunoprecipitation (ChIP)

    Brown Lab IP protocol

3. Yeast DNA mini prep (10 ml)

4. Yeast colony PCR

5. Yeast DNA prep (Large scale 40 ml)

6. Yeast Protein prep for western analysis

7. Yeast plasmid prep for E. coli Transformation

    Clontech protocol to use with column

8. Yeast plasmid prep for yeast transformation

    Total DNA isolation using Qiagen method and then do the transformation.

9. Curing 2 micron DNA

10. RNA isolation (Koshland lab)

11. Haploid yeast cell density

12. DAPI staining of live yeast cells

13. Cell synchronization (using alpha factor)

14. Nocodazole treatment (arrests at G-M phase)

15. Hydroxyl urea arrest

16. FACS analysis using Propidium Iodide

17. Scoring sheets and Grids

            a. Tetrad scoring sheet

            b. Marker/growth scoring sheet

            c. Grid for streaking colonies (small plate)

            d. Streaking grid for bigger plates

18. Yeast immunofluorescence

19. Yeast chromatin spread

20. E. coli Transformation

21. Making E. coli competent cells (RbCl method)

22. Kinasing Oligos

JAYARAM LAB