Modification of the SAG medium. Alkaline media suitable for LB 2340 Spirulina platensis and LB 2342 Spirulina maxima. In order to prevent the formation of precipitates during autoclaving, two solutions are prepared as follows:
For 1 L Total
1. After both solutions 1 and 2 are sterilized, transfer the contents of Solution 2 into the flask containing solution 1, and swirl to mix thoroughly.
2. Store at refrigerator temperature.