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BG-11 Medium  

Directions 
Improved recipe as of March 2009

For 1 L Total

Liquid media:
1. To approximately 900 mL of dH2O add the first 9 components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH2O.
3. Cover and autoclave medium.
4. Allow to cool then store at refrigerator temperature.

Agar media:
1. To approximately 400 mL of dH2O add the first 9 components in the order specified while stirring continuously.
2. Bring total volume to 500 mL with dH2O.
3. In a separate container add 15 g of agar to 500 mL of dH2O.
4. Cover and autoclave both solutions.
5. In a water bath allow both solutions to cool to 45-50°C.
6. Add sterile Sodium Thiosulfate (Component 10) to agar solution and mix well.
7. Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly.
8. Allow to cool then store at refrigerator temperature.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1NaNO3 (Fisher BP360-500)10 mL/L30 g/200 mL dH2O17.6 mM
2K2HPO4 (Sigma P 3786)10 mL/L0.8 g/200 mL dH2O0.23 mM
3MgSO4·7H2O (Sigma 230391)10 mL/L0.15 g/200 mL dH2O0.03 mM
4CaCl2·2H2O (Sigma C-3881)10 mL/L0.72 g/200 mL dH2O0.24 mM
5Citric Acid·H2O (Fisher A 104)10 mL/L0.12 g/200 mL dH2O0.031 mM
6 Ferric Ammonium Citrate10 mL/L0.12 g/200 mL dH2O0.021 mM
7Na2EDTA·2H2O (Sigma ED255)10 mL/L0.02 g/200 mL dH2O0.0027 mM
8Na2CO3 (Baker 3604)10 mL/L0.4 g/200 mL dH2O0.19 mM
9BG-11 Trace Metals Solution1 mL/L  
10Sodium Thiosulfate Pentahydrate (agar media only,sterile) (Baker 3946)1 mL/L49.8 g/200 mL dH2O1 mM

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