Graduate
Student AccomplishmentsRecent Publications
J. Ge and R. M. Harshey. 2008. Congruence of in vivo and in vitro insertion patterns in hot E. coli gene targets of transposable element Mu: Opposing roles of MuB in target capture and integration. J. Mol. Biol. 380: 598-607.
Z. Yin, A. Suzuki, Z. Lou, M. Jayaram and R. M. Harshey. 2007. Interactions of phage Mu enhancer and termini that specify the assembly of a topologically unique interwrapped transpososome. J. Mol. Biol. 372: 382-396.
R. M. Harshey and M. Jayaram. 2006. The Mu transpososome through a topological lens. Crit. Rev. Biochem. Mol. Biol. 41: 387-405.
T. K. Au, P. Agarwal and R. M. Harshey. 2006. Chromosomal integration mechanism of infecting Mu virion DNA. J. Bacteriol. 188: 1829-1834.
Z. Yin and R. M. Harshey. 2005. Enhancer-independent Mu transposition from two topologically distinct synapses. Proc. Natl. Acad. Sci. USA 102: 18884-18889.
Z. Yin, M. Jayaram, S. Pathania and R. M. Harshey. 2005. The Mu transposase interwraps distant DNA sites within a functional transpososome in the absence of DNA supercoiling. J. Biol. Chem. 280: 6149-56.
T. K. Au, S. Pathania and R. M. Harshey. 2004. True reversal of Mu integration. EMBO J. 23: 3408-3420.
I. Lee and R. M. Harshey. 2003. Patterns of sequence conservation at the termini of long terminal repeat (LTR) retrotransposons and DNA transposons in the human genome: Lessons from phage Mu. Nuc. Acids Res. 31: 4531-4540.
S. Pathania, M. Jayaram and R. M. Harshey. 2003. A unique right end-enhancer complex precedes synapsis of Mu ends: The enhancer is sequestered within the transpososome throughout transposition. EMBO J. 22: 3725-3736.
I. Lee and R. M. Harshey. 2003. The conserved CA/TG motif at Mu termini: T specifies stable transpososome assembly. J. Mol. Biol. 330: 261-275.
I. Grainge, S. Pathania, A. Vologodskii, R. M. Harshey, M. Jayaram. 2002. Symmetric DNA sites are functionally asymmetric within Flp and Cre site-specific DNA recombination synapses. J. Mol. Biol., 320: 515-27.
S. Pathania, M. Jayaram and R. M. Harshey. 2002. Path of DNA within the Mu transpososome: Transposase interactions bridging two Mu ends and the transposition enhancer trap five DNA supercoils. Cell, 109: 425-436.
G. Chaconas and R. M. Harshey. 2002. Transposition of phage Mu DNA. In Mobile DNA II. N. L. Craig, R. Craigie, M. Gellert, and A. M. Lambowitz (ed), pp 384-402, American Society for Microbiology.
Selected Other Publications
I. Lee and R. M. Harshey. 2001. Importance of the conserved CA dinucleotide at Mu termini. J. Mol. Biol. 314: 443-454.
H. Jiang, J-Y. Yang and R. M. Harshey. 1999. Criss-crossed interactions between the enhancer and the att sites of phage Mu during DNA transposition. EMBO J. 18: 3845-3855.
S-Y Namgoong and R. M. Harshey. 1998. The same two monomers within a MuA tetramer provide the DDE domains for the strand cleavage and strand transfer steps of transposition. EMBO J. 17: 3775-3785.
Z. Wang, S-Y Namgoong, X. Zhang, and R. M. Harshey. 1996. Kinetic and structural probing of the precleavage synaptic (Type0) formed during phage Mu transposition: Action of metal ions and reagents specific to single-stranded DNA. J. Biol. Chem.271:9619-9626.
J-Y. Yang, M. Jayaram and R. M. Harshey. 1995. Enhancer-independent variants of phage Mu transposase: Enhancer-specific stimulation of catalytic activity by a partner transposase. Genes Dev. 9: 2545-2555.
Z. Wang and R.M. Harshey. 1994. Crucial role for DNA supercoiling in Mu transposition.: A kinetic study. Proc. Natl. Acad. Sci. USA 91: 699-703.
P. C. Leung, D. B. Teplow, and R. M. Harshey. 1989. Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional enhancer. Nature. 338:656-658.
C. Nakayama, D. B. Teplow and R. M. Harshey. 1987. Structural domains in phage Mu transposase: Identification of the site-specific DNA-binding domain. Proc. Natl. Acad. Sci. USA. 84:1809-1813.
R. M. Harshey, 1984. Transposition without duplication of infecting bacteriophage Mu DNA. Nature 311:580-581.
R.
M. Harshey and A. I. Bukhari. 1983.
Infecting bacteriophage Mu DNA
forms a circular DNA-protein complex.
J.
Mol. Biol. 167:427-441.
