Graduate
Student AccomplishmentsRecent Publications
J. S. Jang, S. J. Sandler and R. M. Harshey. 2012. Mu insertions are repaired by the double-strand break repair pathway of E. coli. PLoS Genetics e1002642
J. Ge, Z. Lou, H. Cui L. Shang, and R. M. Harshey. 2011. Analysis of phage Mu DNA transposition by whole-genome Escherichia coli tiling arrays reveals a complex relationship to distribution of target-selection protein B, transcription, and chromosome architectural elements. J. Biosci.36: 587-601.
W. Choi and R. M. Harshey. 2010. DNA repair by the cryptic endonuclease activity of phage Mu transposase. Proc. Natl. Acad. Sci. USA. 107: 10014-10019.
J. Ge, Z. Lou and R. M. Harshey. 2010. Immunity of replicating Mu to self-integration: a novel mechanism employing MuB protein. Mobile DNA, 1(1):8.
M. Jayaram and R. M. Harshey. 2009. Difference topology: Analysis of high-order DNA-protein interactions. In Mathematics of DNA structure, Function and Interactions. Eds. Benham, C.J. et al. Springer, pp 139-158.
J. Ge and R. M. Harshey. 2008. Congruence of in vivo and in vitro insertion patterns in hot E. coli gene targets of transposable element Mu: Opposing roles of MuB in target capture and integration. J. Mol. Biol. 380: 598-607.
Z. Yin, A. Suzuki, Z. Lou, M. Jayaram and R. M. Harshey. 2007. Interactions of phage Mu enhancer and termini that specify the assembly of a topologically unique interwrapped transpososome. J. Mol. Biol. 372: 382-396.
Selected Other Publications
R. M. Harshey and M. Jayaram. 2006. The Mu transpososome through a topological lens. Crit. Rev. Biochem. Mol. Biol. 41: 387-405.
T. K. Au, P. Agarwal and R. M. Harshey. 2006. Chromosomal integration mechanism of infecting Mu virion DNA. J. Bacteriol. 188: 1829-1834.
Z. Yin and R. M. Harshey. 2005. Enhancer-independent Mu transposition from two topologically distinct synapses. Proc. Natl. Acad. Sci. USA 102: 18884-18889.
T. K. Au, S. Pathania and R. M. Harshey. 2004. True reversal of Mu integration. EMBO J. 23: 3408-3420.
I. Lee and R. M. Harshey. 2003. Patterns of sequence conservation at the termini of long terminal repeat (LTR) retrotransposons and DNA transposons in the human genome: Lessons from phage Mu. Nuc. Acids Res. 31: 4531-4540.
S. Pathania, M. Jayaram and R. M. Harshey. 2003. A unique right end-enhancer complex precedes synapsis of Mu ends: The enhancer is sequestered within the transpososome throughout transposition. EMBO J. 22: 3725-3736.
I. Lee and R. M. Harshey. 2003. The conserved CA/TG motif at Mu termini: T specifies stable transpososome assembly. J. Mol. Biol. 330: 261-275.
S. Pathania, M. Jayaram and R. M. Harshey. 2002. Path of DNA within the Mu transpososome: Transposase interactions bridging two Mu ends and the transposition enhancer trap five DNA supercoils. Cell, 109: 425-436.
G. Chaconas and R. M. Harshey. 2002. Transposition of phage Mu DNA. In Mobile DNA II. N. L. Craig, R. Craigie, M. Gellert, and A. M. Lambowitz (ed), pp 384-402, American Society for Microbiology.
I. Lee and R. M. Harshey. 2001. Importance of the conserved CA dinucleotide at Mu termini. J. Mol. Biol. 314: 443-454.
H. Jiang, J-Y. Yang and R. M. Harshey. 1999. Criss-crossed interactions between the enhancer and the att sites of phage Mu during DNA transposition. EMBO J. 18: 3845-3855.
S-Y Namgoong and R. M. Harshey. 1998. The same two monomers within a MuA tetramer provide the DDE domains for the strand cleavage and strand transfer steps of transposition. EMBO J. 17: 3775-3785.
Z. Wang, S-Y Namgoong, X. Zhang, and R. M. Harshey. 1996. Kinetic and structural probing of the precleavage synaptic (Type0) formed during phage Mu transposition: Action of metal ions and reagents specific to single-stranded DNA. J. Biol. Chem.271:9619-9626.
Z. Wang and R.M. Harshey. 1994. Crucial role for DNA supercoiling in Mu transposition.: A kinetic study. Proc. Natl. Acad. Sci. USA 91: 699-703.
P. C. Leung, D. B. Teplow, and R. M. Harshey. 1989. Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional enhancer. Nature. 338:656-658.
C. Nakayama, D. B. Teplow and R. M. Harshey. 1987. Structural domains in phage Mu transposase: Identification of the site-specific DNA-binding domain. Proc. Natl. Acad. Sci. USA. 84:1809-1813.
R. M. Harshey, 1984. Transposition without duplication of infecting bacteriophage Mu DNA. Nature 311:580-581.
R.
M. Harshey and A. I. Bukhari. 1983.
Infecting bacteriophage Mu DNA
forms a circular DNA-protein complex.
J.
Mol. Biol. 167:427-441.
