Wyckoff, E., D. Natalie, J. M. Nolan, M. Lee, and T.-s. Hsieh. 1989. Structure of the Drosophila DNA topoisomerase II gene. Nucleotide sequence and homology among topoisomerases II. J. Mol. Biol. 205:1-13.
We have determined the nucleotide sequence of the Drosophila DNA
topoisomerase II gene. Data from primer extension and S1 nuclease protection
experiments were combined with comparisons of genomic and cDNA sequences to
determine the structure of the mature messenger RNA. This message has a large
open reading frame of 4341 nucleotides. The length of the predicted protein is
1447 amino acids with a molecular weight of 164,424. Topoisomerase II can be
divided into three domains: (1) an N-terminal region with homology to the B (ATPase)
subunit of the bacterial type II topoisomerase, DNA gyrase; (2) a central region
with homology to the A (breaking and rejoining) subunit of DNA gyrase; (3) a
C-terminal region characterized by alternating stretches of positively and
negatively charged amino acids. DNA topoisomerase II from the fruit fly shares
significant sequence homology with those from divergent sources, including
bacteria, bacteriophage T4 and yeasts. The location and distribution of
homologous stretches in these sequences are analyzed.
