Mueller Lab Molecular Protocols

The below are links to commonly used protocols in the Mueller Lab

Mycology

• Cryovialing fungus for long term storage

DNA extraction

• Magic Quick Protocol for Extraction from Garden Material
• Traditional CTAB extraction

PCR

• Traditional PCR for ITS primers
• Notes from Sasha on doing short PCRs (including M13)
• M13-based microsatellite protocol

Sequencing

• PEG, Cycle Sequencing and Sephadex
• Maintaining the ABI 3100 Sequencer
• Cloning

Analysis

• Sequencing Alignment on PCs
• AFLP data organization and scoring