| CLASS |
TOPIC |
| 1. | Introduction to Course |
| 2. | Large-scale preparation of plasmid DNA |
| 3. | Estimation of plasmid DNA yield: digestion with restriction enzyme HindIII and agarose gel electrophoresis. |
| 4. | Mutagenesis of pBR325 with transposon Tn5; transformation |
| 5. | Isolation of pBR325::Tn5 DNA: plasmid mini-preps |
| 6. | Restriction mapping of Tn5 insertions |
| 7. | Restriction mapping (continued) |
| 8. | FIRST PAPER DUE. Deletion mutagenesis in vitro with Ba131 exonuclease |
| 9. | DNA amplification with PCR |
| 10. | Cloning; polyacrylamide gel electrophoresis |
| 11. | Transfection with M13 recombinant DNA |
| 12. | Preparation of single-stranded M13 phage DNA |
| 13. | DNA sequencing (I) |
| 14. | DNA sequencing (II) |
| 15. | SECOND PAPER DUE; clean-up |
School of Biological
Sciences | College of Natural Science
The University of Texas at Austin |
Biological Science Computer Support
Facility